Isolation of Salmonella Typhimurium and Detection of Virulence Contributing Genes from Water of River Ganges
River Ganges is one of the most important rivers of India. This important and sacred river is becoming contaminated day by day. In the present study, 500 water samples were collected from river Ganges and screened for presence of Salmonella by PCR. Out of 500 samples, 44 were found to be positive for Salmonella and Salmonella enterica serovar Typhimurium was the most common serovar isolated from 24 samples. These isolates were screened for presence of stn, inv A and pef A genes which are mainly responsible for virulence of Salmonella. In 21 isolates stn gene was present, while inv A and pef A genes were present in 22 and 18 isolates respectively. In 15 isolates, all three virulent genes were present. The present study indicates that these isolates may be virulent and being a serious cause of health problems both in animals and humans.
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