In recent decades, the utilization of energy-based devices, fillers, toxins, and threads for aesthetic intentions has seen a significant increase, becoming a common practice in clinical settings. Nonetheless, there remains a dearth of precise information regarding appropriate injection techniques and anatomical considerations. While these clinical procedures, both in therapeutic and cosmetic contexts, have gained popularity, there is a notable lack of comprehensive understanding, leading to the documentation of various adverse effects. The underlying causes of these undesirable outcomes are attributed not solely to improper injection methods but also to an inadequate grasp of the relevant anatomy, contributing to inconsistent clinical results. This Special Issue aims to provide comprehensive guidelines for both anatomical accuracy and aesthetic goals, ensuring safe and effective treatments. The publication will evaluate the most up-to-date insights from anatomical research, aiding in the identification of optimal approaches for applications. Additionally, the integration of clinically guided methodologies, such as ultrasonography, along with novel injection techniques utilizing diverse equipment, is encouraged and embraced.