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REVIEW ARTICLE

Single-cell sequencing for lung cancer research: Progress and prospects

Zhongsha Li1,2,3,4† ,  Wei Su1,2,3,4† ,  Bingbing Bai1,2,3,4† ,  Yuesong Wu1,2,3,4 ,  Baoyin Zhang1,2,3,4 ,  Dejuan Xiang1,2,3,4 ,  Gilwa Borjigin1,2,3,4 ,  Jinhai Deng1,2,3,4,5 ,  Jian Sun1,2,3,4†* ,  Mingzhu Yin1,2,3,4†*
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1 Clinical Research Center, Medical Pathology Center, Cancer Early Detection and Treatment Center and Translational Medicine Research Center, Chongqing University Three Gorges Hospital, Chongqing University, Wanzhou District, Chongqing, China
2 Chongqing Technical Innovation Center for Quality Evaluation and Identification of Authentic Medicinal Herbs, Wanzhou District, Chongqing, China
3 Chongqing University Three Gorges Hospital and Academy for Advanced Interdisciplinary Technology, CQU-Ferenc Krausz Nobel Laureate Scientific Workstation, Chongqing, China
4 School of Medicine Chongqing University, Chongqing University, Shapingba District, Chongqing, China
5 Richard Dimbleby Department of Cancer Research, Comprehensive Cancer Centre, Kings College London, London, United Kingdom
EJMO, 6883 https://doi.org/10.36922/ejmo.6883
Submitted: 3 December 2024 | Revised: 12 February 2025 | Accepted: 27 February 2025 | Published: 12 March 2025
© 2025 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution -Noncommercial 4.0 International License (CC-by the license) ( https://creativecommons.org/licenses/by-nc/4.0/ )
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Abstract

Lung cancer exhibits high morbidity and mortality rates, characterized by a heterogeneous genetic landscape and an immunosuppressive tumor microenvironment, despite recent therapeutic advances. High-resolution single-cell sequencing has facilitated a comprehensive analysis of malignant and host cell types, enhancing the understanding of lung cancer’s heterogeneity and adaptability under basal conditions and in response to therapeutic intervention. In this review, we provide an overview of the utilization of single-cell sequencing technology in characterizing the microenvironment of lung cancer, facilitating early diagnosis, monitoring tumor progression, and elucidating mechanisms of drug resistance, thereby offering new insights into potential therapeutic interventions.

Keywords
Single-cell sequencing
Lung cancer
Heterogeneity
Tumor microenvironment
Drug resistance
Circulating tumor cells
Funding
This work was supported by the National Natural Science Foundation of China (No. 82200070), the Chongqing Wanzhou Municipal Science and Health Joint Research Project (Grant No. wzwjw-kw2024008), the Chongqing Wanzhou Municipal Science and Health Joint Medical Research Project Key Program (Grant No. wzstc-kw2023001), the Innovation and Development Joint Fund of the Natural Science Foundation of Chongqing (No. CSTB2023NSCQ-LMX0037 and No. CSTB2023NSCQ-LMX0038), and the Natural Science Foundation of Chongqing, China (No. CSTB2024NSCQ-MSX0761).
Conflict of interest
The authors declare no conflicts of interest.
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Eurasian Journal of Medicine and Oncology, Electronic ISSN: 2587-196X Print ISSN: 2587-2400, Published by AccScience Publishing
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