Ghrelin Inhibit the Synovial Cells Apoptosis and Aoutphagy Via ADORA2B/ PI3K/Akt/mTOR Axis
Objectives: To explore the regulatory role of Ghrelin in synovial cells from OA patient apoptosis and autophagy in as-sociation with ADORA2B and PI3K/Akt/mTOR signaling pathway.
Methods: Ghrelin was detected by elisa kit in OA patient. And the cell proliferation was determined by CCK-8. The protein expression such as LC3 II/I, Becline-1, Bax, Bcl-2, p-PI3K, p-Akt, p-mTOR , PI3K, Akt, mTOR, GAPDH was detected by western blot.
Results: In the OA patients, ghrelin was high expression in the serum. Ghrelin could promote the proliferation of the synovial cells, and inhibit the synovial cells apoptosis and aoutphagy. The LC3 II/I, Becline-1 and Bax was lower and the Bcl-2 was higher in the ghrelin group than the control group. And this processing was regulated by ADORA2B. And the ADORA2B could regulated the ghrelin inhibiting the synovial cells apoptosis and aoutphagy though PI3k/Akt/mTOR pathway.
Conclusion: Ghrelin could inhibit the synovial cells apoptosis and aoutphagy via ADORA2B/ PI3K/Akt/mTOR axis.
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