AccScience Publishing / AN / Volume 2 / Issue 3 / DOI: 10.36922/an.334
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ORIGINAL RESEARCH ARTICLE

Transient receptor potential melastatin 7 signaling in U251 cell migration and invasion involves calcineurin

Haifan Gong1 ,  Raymond x Wong1 ,  Julia Bandura1 ,  James T. Rutka2 ,  Zhong-Ping Feng1* ,  Hong-Shuo Sun1,2,3,4*
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1 Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
2 Department of Surgery, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
3 Department of Pharmacology and Toxicology, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
4 Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario, Canada
Advanced Neurology 2023, 2(3), 334 https://doi.org/10.36922/an.334
Submitted: 19 January 2023 | Accepted: 10 July 2023 | Published: 24 July 2023
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Transient receptor potential melastatin 7 (TRPM7) is a divalent cation channel that has crucial functions in glioblastoma (GBM), which remains the most prevalent and lethal primary brain tumor in adults. Altering TRPM7 activity has previously been reported to affect GBM cell function (i.e., migration, invasion, and proliferation), thus elucidating the TRPM7-mediated signaling pathway in GBM could reveal novel therapeutic targets. Calcineurin, a Ca2+-dependent phosphatase, also influences GBM cell survival and migration. However, the role or the relationship between TRPM7 and calcineurin in GBM signaling has not previously been investigated. In this study, we provide evidence that there is a possible interaction between TRPM7 and calcineurin in the GBM cell line U251. Moreover, we employed pharmacological approaches to show that TRPM7 regulates calcineurin function, thereby suggesting that calcineurin is a potential downstream target of TRPM7 signaling in U251 cell migration and invasion.

Keywords
Glioblastoma
Transient receptor potential melastatin 7 channels
Calcineurin
Funding
Canadian Institutes of Health Research
Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grants
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Conflict of interest
The authors declare that they have no conflicts of interest with the contents of this article.
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Advanced Neurology, Electronic ISSN: 2810-9619 Published by AccScience Publishing
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