Spatial assessment of water quality in a hierarchically structured river system using stream order classification and multivariate statistical techniques: A case study from Tunduma, Tanzania
Water quality assessment is essential for understanding pollutant dynamics, supporting evidence-based watershed management, and protecting public health. While numerous studies have utilized statistical and modeling approaches, limited attention has been paid to how stream order influences water quality variability, particularly in urban catchments of sub-Saharan Africa. This study investigates spatial patterns of water quality in a hierarchically structured stream network in Tunduma, Tanzania, by integrating Strahler stream order classification with multivariate statistical techniques, based on monthly monitoring of six surface water points over 12 months (n = 72) during both wet and dry seasons to analyze physicochemical, nutrient, and microbial parameters. Hierarchical cluster analysis, combined with Pearson correlation matrices and significance testing, was employed to assess pollutant similarity and accumulation patterns across different stream orders. Results revealed that phosphate (PO43−) concentrations ranged from 0.42 to 1.49 mg/L and nitrate (NO3−) levels ranged from 4.3 to 13.2 mg/L. Strong positive correlations (r > 0.95) were observed among ion-derived parameters, such as electrical conductivity, total dissolved solids, and the concentrations of calcium (Ca2+) and magnesium (Mg2+). Third-order stream segments exhibited elevated concentrations of total suspended solids (0.990), biochemical oxygen demand (0.982), and microbial indicators, with fecal coliforms of 0–5 CFU/100 mL and total coliforms of 0–18 CFU/100 mL, reflecting cumulative pollutant loading in downstream reaches. The integration of Strahler stream ordering and cluster-based analytics enabled the identification of pollution hotspots and revealed the critical role of hydrological connectivity in shaping water quality trends. This research contributes a novel spatial–statistical framework for stream-based water quality assessment in East African urban contexts, offering practical insights for catchment-scale pollution control and resource management.

- Zheng LY, Yu HB, Wang QS. Assessment of temporal and spatial variations in surface water quality using multivariate statistical techniques: A case study of Nenjiang River basin, China. J Cent South Univ. 2015;22(10):3770-3780. doi: 10.1007/s11771-015-2921-z
- Siriwardhana KD, Jayaneththi DI, Herath RD, et al. A simplified equation for calculating the water quality index (WQI), Kalu River, Sri Lanka. Sustainability. 2023;15(15):12012. doi: 10.3390/su151512012
- Meyer JL, Paul MJ, Taulbee WK. Stream ecosystem function in urbanizing landscapes. J North Am Benthol Soc. 2005;24(3):602-612. doi: 10.1899/04-021.1
- Duan W, He B, Nover D, et al. Water quality assessment and pollution source identification of the eastern poyang lake basin using multivariate statistical methods. Sustainability. 2016;8(2):133. doi: 10.3390/su8020133
- Wu Y, Giri S, Qiu Z. Understanding the spatial distribution of hydrologic sensitive areas in the landscape using soil topographic index approach. Int Soil Water Conserv Res. 2016;4(4):278-283. doi: 10.1016/j.iswcr.2016.10.002
- Payus C, Ann Huey L, Adnan F, et al. Impact of extreme drought climate on water security in north borneo: case study of sabah. Water (Basel). 2020;12(4):1135. doi: 10.3390/w12041135
- Hellar-Kihampa H, De Wael K, Lugwisha E, Van Grieken R. Water quality assessment in the Pangani River basin, Tanzania: Natural and anthropogenic influences on the concentrations of nutrients and inorganic ions. Int J River Basin Manag. 2013;11(1):55-75. doi: 10.1080/15715124.2012.759119
- Chen L, Han L, Tan J, et al. Water environmental capacity calculated based on point and non-point source pollution emission intensity under water quality assurance rates in a Tidal river network area. Int J Environ Res Public Health. 2019;16(3):428. doi: 10.3390/ijerph16030428
- Alexander RB, Boyer EW, Smith RA, Schwarz GE, Moore RB. The role of headwater streams in downstream water quality. J Am Water Resour Assoc. 2007;43(1):41-59. doi: 10.1111/j.1752-1688.2007.00005.x
- Ensign SH, Doyle MW. Nutrient spiraling in streams and river networks. J Geophys Res Biogeosci. 2006;111(G4). doi: 10.1029/2005JG000114
- Makumbura RK, Mampitiya L, Rathnayake N, et al. Advancing water quality assessment and prediction using machine learning models, coupled with explainable artificial intelligence (XAI) techniques like shapley additive explanations (SHAP) for interpreting the black-box nature. Results Eng. 2024;23:102831. doi: 10.1016/j.rineng.2024.102831
- Rathnayake N, Rathnayake U, Chathuranika I, Dang TL, Hoshino Y. Cascaded-ANFIS to simulate nonlinear rainfall-runoff relationship. Appl Soft Comput. 2023;147:110722. doi: 10.1016/j.asoc.2023.110722
- Kiss T, Fiala K, Sipos G, Szatmári G. Long-term hydrological changes after various river regulation measures: Are we responsible for flow extremes? Hydrol Res. 2019;50(2):417-430. doi: 10.2166/nh.2019.095
- Mbululo Y, Nyihirani F. Climate characteristics over Southern highlands Tanzania. Atmos Clim Sci. 2012;2(4):454-463. doi: 10.4236/acs.2012.24039
- William M, Katambara Z. Assessment of spatial water quality variations in shallow wells using principal component analysis in half London Ward, Tanzania. J Water Resour Prot. 2025;17(2):108-143. doi: 10.4236/jwarp.2025.172007
- VanDerWal J, Shoo LP, Graham C, Williams SE. Selecting pseudo-absence data for presence-only distribution modeling: How far should you stray from what you know? Ecol Modell. 2009;220(4):589-594. doi: 10.1016/j.ecolmodel.2008.11.010
- Goyal VC, Jain SK, Pareek N. Water logging and drainage Assessment in Ravi-Tawi irrigation command (J&K) using remote sensing approach. J Indian Soc Remote Sens. 2005;33(1):7-15. doi: 10.1007/BF02989986
- Cabral S, Campos JNB, Silveira C. hydrologic and hydraulic modelling integrated with gis: A study of the Acaraú River basin – CE. J Urban Environ Eng. 2015;8:167-174. doi: 10.4090/juee.2014.v8n2.167-174
- Edokpayi JN, Rogawski ET, Kahler DM, et al. Challenges to sustainable safe drinking water: A case study of water quality and use across seasons in rural communities in Limpopo Province, South Africa. Water. 2018;10(2):159. doi: 10.3390/w10020159
- Du X, Shao F, Wu S, Zhang H, Xu S. Water quality assessment with hierarchical cluster analysis based on Mahalanobis distance. Environ Monit Assess. 2017;189(7):335. doi: 10.1007/s10661-017-6035-y
- Athamena A, Gaagai A, Aouissi HA, et al. Chemometrics of the environment: Hydrochemical characterization of groundwater in Lioua Plain (North Africa) using time series and multivariate statistical analysis. Sustainability. 2022;15(1):20. doi: 10.3390/su15010020
- Lapworth DJ, Nkhuwa DCW, Okotto-Okotto J, et al. Urban groundwater quality in sub-Saharan Africa: Current status and implications for water security and public health. Hydrogeol J. 2017;25(4):1093-1116. doi: 10.1007/s10040-016-1516-6
- Vannote RL, Minshall GW, Cummins KW, Sedell JR, Cushing CE. The river continuum concept. Can J Fish Aquat Sci. 1980;37(1):130-137. doi: 10.1139/f80-017
- Uygur A, Kargı F. Biological nutrient removal from pre-treated landfill leachate in a sequencing batch reactor. J Environ Manage. 2004;71(1):9-14. doi: 10.1016/j.jenvman.2004.01.002
- Withers PJA, Jarvie HP. Delivery and cycling of phosphorus in rivers: A review. Sci Total Environ. 2008;400(1-3):379-395. doi: 10.1016/j.scitotenv.2008.08.002
- Alavaisha E, Lyon SW, Lindborg R. Assessment of water quality across irrigation schemes: A case study ofwetland agriculture impacts in Kilombero Valley, Tanzania. Water (Switzerland). 2019;11(4):671. doi: 10.3390/w11040671
- Beaulieu JJ, Tank JL, Hamilton SK, et al. Nitrous oxide emission from denitrification in stream and river networks. Proc Natl Acad Sci. 2011;108(1):214-219. doi: 10.1073/pnas.1011464108
- Lam QD, Schmalz B, Fohrer N. Assessing the spatial and temporal variations of water quality in lowland areas, Northern Germany. J Hydrol (Amst). 2012;438-439:137-147. doi: 10.1016/j.jhydrol.2012.03.011
- Fang H, Vergara GGR, Goh SG, Ang CYL, Gu X, Gin KY. Effect of rainfall on the microbial water quality of a tropical Urban catchment. J Environ Qual. 2018;47(5):1242-1248. doi: 10.2134/jeq2018.03.0099
- Shrestha S, Kazama F. Assessment of surface water quality using multivariate statistical techniques: A case study of the Fuji river basin, Japan. Environ Modell Softw. 2007;22(4):464-475. doi: 10.1016/j.envsoft.2006.02.001
- Dodds WK, Oakes RM. Headwater influences on downstream water quality. Environ Manage. 2008;41(3):367-377. doi: 10.1007/s00267-007-9033-y
