Biological Denitrification of Ground Water Using Various Carbon Sources by Pseudomonas Fluorescens and Pseudomonas Stutzeri in a Hetrotrophic Denitrification Reactor

Archna ^1*, R.C. Sobti², d S.K. Sharma⁴

Show Less

¹ Department of Chemical Engineering, MS Ramaiah Institute of Technology, Bangalore – 560054

² Department of Biotechnology, Panjab University, Chandigarh – 160014

³ Department of Chemical Engineering, Panjab University, Chandigarh –160014

AJWEP 2011, 8(4), 21–25; https://doi.org/10.3233/AJW-2011-8_4_04

Submitted: 1 March 2011 | Accepted: 2 June 2011 | Published: 1 January 2011

© 2011 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution -Noncommercial 4.0 International License (CC-by the license) ( https://creativecommons.org/licenses/by-nc/4.0/ )

Download PDF

Cite

XML

Abstract

A pilot plant study has been performed on biological removal of nitrate using heterotrophic denitrification reactor. The system behaviour has been analysed with the usage of – (i) three carbon sources (cotton, wheat straw and wood shavings), (ii) two microbial strains (Pseudomonas fluorescens NCIM 5059 and Pseudomonas stutzeri NCIM 5136) and (iii) at different temperatures. An optimal operating mode for 90% nitrate removal has been achieved by Pseudomonas fluorescens with inlet nitrate concentration of 200 ppm, flow rate 2.5 ml/min, carbon source cotton, temperature 30°C±0.5°C and for Pseudomonas stutzeri at same process conditions but with the temperature of 40°C±0.5°C. The feasibility of nitrate removal was also tested with carbon source as wood shavings and wheat straw.

Keywords

Denitrification

heterotrophic denitrification reactor (HDR)

trickling sand filter (TSF)

solid natural carbon source

Conflict of interest

The authors declare they have no competing interests.

References

Della Rocca, C. (2005). Biological Denitrification of Drinking Water: Cotton and ZVI Supported Heterotrophic-Autotrophic Denitrification of Nitrate Rich Potable Water. Ph.D. Thesis, University of Salerno, Engineering Faculty.

Janos Sandor, Istvan Kiss, Orsolya Farkas and Istvan Ember (2001). Association between gastric cancer mortality and nitrate content of drinking water: Ecological study on small area inequalities. European Journal of Epidemiology, 17: 443–447.

Kapoor, A. and T. Viraraghavan (1997). Nitrate removal from drinking water – review. Environ. Eng., 123: 371–380.

Mary Ann Robinson-Lora and Rachel A. Brennan (2009). The use of crab-shell chitin for biological denitrification: Batch and column tests. Biosource Technology, 100: 534–541.

Mary H. Ward, Theo M. deKok, Patrick Levallois, Jean Brender, Gabriel Gulis, Bernard T. Nolan and James VanDerslice (2005). Drinking Water Nitrate and Health – Recent Findings and Research Needs. Environ. Health Perspect, 113: 1607–1614.

Mateju, V., Cizinska, S., Krejei, J. and T. Janoch (1992). Biological water denitrification: A review. Enzyme Microbe Technol., 14: 170–183.

Metcalf and Eddy (2003). Denitrification. In: Waste water engineering treatment disposal and reuse, 4th edn. Tata McGraw Hill, New Delhi.

Oskar Modin, Kensuke Fukushi and Kazuo Yamamoto (2007). Denitrification with methane as external carbon source. Water Research, 41: 2726–2738.

Ovez, S. Ozgen and M. Yuksel (2006). Biological denitrification in drinking water using Glycyrrhiza glabra and Arunda donax as the carbon source. Process Biochemistry, 41: 1539–1544.

Ovez Bikem (2006). Batch biological denitrification using Arundo donax, Glycyrrhiza glabra, and Gracilaria verrucosa as carbon source. Process Biochemistry, 41: 1289–1295.

Prashant M. Biradar, Roy, S.B., D’Souza, S.F. and A.B. Pandit (2010). Excess cell mass as an internal carbon source for biological denitrification. Biosource Technology, 101: 1787–1791.

Soares, M.I.M. (2000). Biological denitrification of ground water. Water Air Soil Pollut., 123: 183–193.

Soares, M.I.M., Belkim, S. and A. Abeliovich (1988). Biological ground water denitrification; laboratory studies. Wat. Sci. Technol., 20: 189–195.

Willie, Jones, Saliling, B., Westerman, Philip W. and Thomas M. Losordo (2007). Wood chips and wheat straw as alternative biofilter media for denitrification reactors treating aquaculture and other wastewaters with high nitrate concentrations. Aquacultural Engineering, 37: 222–233.

Xu, Zu-xin, Shao, Liu, Yin, Hai-long, Chu, Hua-qiang and Yi-jun Yao (2009). Biological Denitrification Using Corncobs as a Carbon Source and Biofilm Carrier. Water Environment Research, 81: 242–247.

Previous article in this issue

Next article in this issue

Asian Journal of Water, Environment and Pollution, Electronic ISSN: 1875-8568 Print ISSN: 0972-9860, Published by AccScience Publishing