Microbial Denitrification of Ground Water – Batch Study
Nitrate pollution of ground water is increasing alarmingly in various countries. Biological denitrification has been found as the most inexpensive and effective technique for nitrate removal. The present work involves batch studies for heterotrophic biological denitrification using cotton as the carbon source and Pseudomonas fluorescens NCIM 5059 and Pseudomonas stutzeri NCIM 5136 as the microbial strains. A maximum removal of 91.0 mg/l at 30 ºC for the strain Pseudomonas fluorescens and 89.2 mg/l at 40 ºC for the strain Pseudomonas stutzeri was observed for an initial nitrate concentration of 100 mg/l. Both the organisms followed Monods Growth Kinetics with μmax values of 5.88 days-1 for Pseudomonas fluorescens and 5 days–1 for Pseudomonas stutzeri, respectively.
Janos Sandor, Istvan Kiss, Orsolya Farkas and Istvan Ember (2001). Association between gastric cancer mortality and nitrate content of drinking water: Ecological study on small area inequalities. European Journal of Epidemiology, 17: 443–447.
Kapoor, A. and T. Viraraghavan (1997). Nitrate removal from drinking water – Review, Environ. Eng., 123: 371–380. Karnataka Rural Water Supply and Sanitation Agency (KRWSSA) Report, 2000.
Mary Ann Robinson-Lora and Rachel A. Brennan (2009). The use of crab-shell chitin for biological denitrification: Batch and column tests. Biosource Technology, 100: 534–541.
Oskar Modin, Kensuke Fukushi and Kazuo Yamamoto (2007). Denitrification with methane as external carbon source. Water Research, 41: 2726–2738.
Ovez, B., Ozgen, S. and M. Yuksel (2006). Biological denitrification in drinking water using Glycyrrhiza glabra and Arunda donax as the carbon source. Process Biochemistry, 41: 1539–1544.
Ovez Bikem (2006). Batch biological denitrification using Arundo donax, Glycyrrhiza glabra and Gracilaria verrucosa as carbon source. Process Biochemistry, 41: 1289–1295.
Prashant M. Biradar, Roy, S.B., D’Souza, S.F. and A.B. Pandit (2010). Excess cell mass as an internal carbon source for biological denitrification. Biosource Technology, 101: 1787–1791.
Soares, M.I.M. (2000). Biological denitrification of ground water. Water Air Soil Pollut., 123: 183–193.
Willie, Jones B., Saliling, Philip W. Westerman and Thomas M. Losordo (2007). Wood chips and wheat straw as alternative biofilter media for denitrification reactors treating aquaculture and other wastewaters with high nitrate concentrations. Aquacultural Engineering, 37: 222–233.
WHO (2003). Guidelines for Drinking-water Quality (3rd edn.), World Health Organization.
Xu, Zu-xin, Shao, Liu, Yin, Hai-long, Chu, Hua-qiang and Yi-jun Yao (2009). Biological Denitrification Using Corncobs as a Carbon Source and Biofilm Carrier, Water Environment Research, 81: 242–247.