Molecular Detection of Group C Rotavirus in  Environmental Samples in Giza, Egypt

Mohamed N.F. Shaheen; Elmahdy M. Elmahdy

doi:10.3233/AJW190044

Group C rotavirus (RVC) has been identified as important enteric pathogen associated with acute gastroenteritis in human, especially in outbreaks. To evaluate the presence of RVC in environmental samples, one-year study was performed in Giza, Egypt. A total of 64 sewage (raw and treated) with 32 sewage sludge samples from Zenin wastewater treatment plant (WWTP) and 72 drainage water with 24 sediment samples from El-Rahawy drain which receives the sewage effluent of Zenin WWTP were collected. After concentration of the collected samples, semi-nested RT-PCR using specific primers was used for amplification of the RVC VP7 gene. The overall detection rates were 21.9% (7 of 32) for the raw sewage, 6% (2 of 32) for the treated sewage, 12.5% (4 of 32) for the sewage sludge, 22% (16 of 72) for the drainage water, and 16.6% (4 of 24) for the drainage sediment. In addition, the virus was abundant in cold months (October-March) and less prevalent in hot months (April-September). The widespread occurrence of RVC in this study reflects that RVC circulates at a relatively high frequency in the Egyptian population.

Anderson, E.J. and S.G. Weber (2004). Rotavirus infections in adults. Lancet Infect. Dis., 4, 91-99.

Chitambar, S., Gopalkrishna, V., Chhabra, P., Patil, P., Verma, H., Lahon, A., Arora, R., Tatte, V., Ranshing, S., Dhale, G. and R. Kolhapure (2012). Diversity in the enteric viruses detected in outbreaks of gastroenteritis from Mumbai, Western India. International Journal of Environmental Research and Public Health, 9(3), 895-915.

El-Senousy, W.M., Ragab, A.M.E.S. and E.M.A.E.H. Handak (2015). Prevalence of rotaviruses groups A and C in Egyptian children and aquatic environment. Food and Environmental Virology, 7(2), 132-141.

EPA – Environmental Protection Agency (1992). Standards for the disposal of sewage sludge, federal register. Washington, 503, 9387-9404.

Estes, M.K. and H.B. Greenberg (2013). Rotavirus. In: Knipe, D.M. and Howley, P.M. (eds). Fields Virology. Philadelphia: Lippincott Williams & Wilkins, Inc.

Gabbay, Y.B., Jiang, B., Oliveira, C.S., Mascarenha s, J.D., Leite, J.P.G., Glass, R.I. and A.C. Linhares (1999). An outbreak of group C rotavirus gastroenteritis among children attending a day-care center in Belem, Brazil. J. Diarrhoeal Dis. Res., 2, 69-74.

Gentsch, J.R., Laird, A.R., Bielfelt, B, Griffin, D.D., Bányai, K., Ramachandran, M, Jain, V, Cunliffe, N.A., Nakagomi, O, Kirkwood, C.D. and T.K. Fischer (2005). Serotype diversity and reassortment between human and animal rotavirus strains: Implications for rotavirus vaccine programs. Journal of Infectious Diseases, 192(1), S146-S159.

Guo, Z., Huang, J, Shi, G, Su, C.H. and J.J. Niu (2014). A food-borne outbreak of gastroenteritis caused by norovirus GII in a university located in Xiamlet City, China. International Journal of Infectious Diseases, 28, 101-106.

Ishimaru, Y., Nakano, S, Nakano, H, Oseta, M. and Y. Yamashita (1991). Epidemiology of group C rotavirus in Matsuyama, Japan. Acta Paediatr. Jpn., 33, 50-56.

James, V.L.A., Lambden, P.R., Caul, E.O., Cooke, S.J. and I.N. Clarke (1997). Seroepidemiology of human group C rotavirus in the UK. J. Med. Virol., 52, 86-91.

Katayama, H, Shimasaki, A. and S. Ohgaki (2002). Development of a virus concentration method and its application to detection of enterovirus and Norwalk virus from coastal seawater. Applied and Environmental Microbiology, 68, 1033-1039.

Kukkula, M, Arstila, P, Klossner, M.L., Maunula, L, Bonsdorff, C.H.V. and P. Jaatinen (1997). Waterborne outbreak of viral gastroenteritis. Scand. J. Infect. Dis., 29, 415-418.

Kumazaki, M. and S. Usuku (2014). Epidemiological and genetic analysis of human group C rotaviruses isolated from outbreaks of acute gastroenteritis in Yokohama, Japan, between 2006 and 2012. Archives of Virology, 159(4), 761-771.

Kuzuya, M, Fujii, R, Hamano, M, Ohata, R, Ogura, H. and M. Yamada (2001). Seroepidemiology of human group C rotavirus in Japan based on blocking enzyme-linked immunosorbent assay. Clin. Diagn. Lab. Immunol., 8, 161-165.

Kuzuya, M, Fujii, R, Hamano, M, Yamada, M, Shinozaki, K, Sasagawa, A, Hasegawa, S, Kawamoto, H, Matsumoto, K, Kawamoto, A. and A. Itagaki (1998). Survey of human group C rotaviruses in Japan during the winter of 1992 to 1993. J. Clin. Microbiol., 36, 6-10.

Lanata, C.F., Fischer-Walker, C.L, Olascoaga, A.C., Torres, C.X, Aryee, M.J. and R.E. Black Child Health Epidemiology Reference Group of the World Health Organization and UNICEF (2013). Global causes of diarrheal disease mortality in children <5 years of age: A systematic review. PloS ONE, 8, e72788.

Liu, L, Oza, S, Hogan, D, Perin, J, Rudan, I, Lawn, J.E, Cousens, S, Mathers, C. and R.E. Black (2015). Global, regional, and national causes of child mortality in 2000–13, with projections to inform post-2015 priorities: An updated systematic analysis. Lancet, 385, 430-440.

Matthijnssens, J, Otto, P.H, Ciarlet, M, Desselberger, U, Van Ranst, M. and R. Johne (2012). VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation. Arch Virol., 157, 1177-1182.

Meleg, E, Bányai, K, Martella, V, Jiang, B, Kocsis, B, Kisfali, P, Melegh, B. and G. Szűcs (2008). Detection and quantification of group C rotaviruses in communal sewage. Applied and Environmental Microbiology, 74(11), 3394-3399.

Mihalov-Kovács, E, Gellért, Á, Marton, S, Farkas, S.L, Fehér, E, Oldal, M, Jakab, F, Martella, V. and K. Bányai (2015). Candidate new rotavirus species in sheltered dogs, Hungary. Emerg. Infect. Dis., 21, 660-663.

Moresco, V, Damazo, N.A. and C.R.M. Barardi (2016). Thermal and temporal stability on the enteric viruses infectivity in surface freshwater. Water Science and Technology: Water Supply, 16(3), 620-627.

Myrmel, M, Berg, E.M, Grinde, B. and E. Rimstad (2006). Enteric viruses in inlet and outlet samples from sewage treatment plants. J. Water Health, 4, 197-209.

Nilsson, M. and L. Svensson (1993). Antibody prevalence and specificity against group C rotavirus. In: Glasgow: Program and Abstracts of the IXth International Congress of Virology.

Nilsson, M, Svenungsson, B, Hedlund, K.O, Uhnoo, I, Lagergren, A, Akre, T. and L. Svensson (2000). Incidence and genetic diversity of group C rotavirus among adults. The Journal of Infectious Diseases, 182(3), 678-684.

Offit, P.A. and H.F. Clark (2000). Rotavirus. In: G.L. Mandel, J.E. Bennet and R. Dolin (eds), Principles and practice of infectious diseases, 5th ed. Churchill Livingstone, New York, NY.

Patton, J.T. (2012). Rotavirus diversity and evolution in the postvaccine world. Discov. Med., 13, 85-97.

Pereira, H.G., Linhares, A.C, Candeias. J.A.N. and R.I. Glass (1993). National laboratory surveillance of viral agents of gastroenteritis in Brazil. Bull. Pan. Am. Health Organ, 27, 224-233.

Rutjes, S.A., Lodder, W.J., Van Leeuwen, A.D. and A.M. de Roda Husman (2009). Detection of infectious rotavirus in naturally contaminated source waters for drinking water production. Journal of Applied Microbiology, 107(1), 97-105.

Schlindwein, A.D., Rigotto, C., Simões, C.M.O. and C.R.M. Barardi (2010). Detection of enteric viruses in sewage sludge and treated wastewater effluent. Water Science and Technology, 61(2), 537-544.

Steele, A.D. and V.L.A. James (1999). Seroepidemiology of human group C rotavirus in South Africa. J. Clin. Microbiol., 37, 4142-4144.

Steyer, A., Poljšak-Prijatelj, M., Bufon, T., Sedmak, M., Vidmar, L., Mijovski, J.Z. and J. Marin (2006). First detection of group C rotavirus in patients with gastroenteritis in Slovenia. Journal of Medical Virology, 78(9), 1250-1255.

Tiku, V.R., Jiang, B., Kumar, P., Aneja, S., Bagga, A., Bhan, M.K. and P. Ray (2017). First study conducted in Northern India that identified group C rotavirus as the etiological agent of severe diarrhea in children in Delhi. Virology Journal, 14(1), 100.

USEPA (2011). Manual of methods for virology. EPA/600/4-84/013. USEPA, Cincinnati, USA