AccScience Publishing / AJWEP / Volume 12 / Issue 4 / DOI: 10.3233/AJW-150020
RESEARCH ARTICLE

Effective Utilization of Leather Waste for Cultivation of Bacteria

Rajendran Kumar1* Swarna V. Kanth2 V. Sasi1 G. Jagan1 Shampa Sen1
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1 Industrial Biotechnology Division, School of Bio Sciences and Technology VIT University, Vellore, Tamil Nadu – 632014, India
2 Central Leather Research Institute, Council of Scientific and Industrial Research Adyar, Chennai, Tamil Nadu – 600020, India
AJWEP 2015, 12(4), 79–82; https://doi.org/10.3233/AJW-150020
Submitted: 9 March 2015 | Revised: 5 October 2015 | Accepted: 5 October 2015 | Published: 1 January 2015
© 2015 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution -Noncommercial 4.0 International License (CC-by the license) ( https://creativecommons.org/licenses/by-nc/4.0/ )
Abstract

Environmental pollution is one of the major problems associated with rapid industrialization in  developing countries. Tanneries generate huge amount of solid waste. Leather waste has been utilized for land  filling, preparation of boards, soil fertilizer and animal feed. In the present study, solid leather waste was hydrolyzed  with acid and alkali. The tanning agent was removed from the hydrolysate. The hydrolysate was analyzed for  protein/amino acid content. The hydrolysate along with agar was used as solid media (Leather Hydrolysate Agar)  for the cultivation of Staphylococcus aureus and Escherichia coli. The significant growth of bacteria on leather  hydrolysate agar shows the possible use of leather waste hydrolysate in the preparation of microbiological media  as well as supplement to bacteriological media.

Keywords
Leather waste
hydrolysis
leather hydrolysate agar
bacteriological media
Conflict of interest
The authors declare they have no competing interests.
References
Alexander, K.T.W., Corning, D.R., Cory, N.J., Donohue, V.J. and R.L. Sykes (1991). Environmental and safety issues clean technology and environmental auditing. J. Soc. Leather Technol. Chem., 76(1): 17-23.

Aspmo, S.I., Horn, S.J. and V.G.H. Eijsink (2005). Hydrolysates from Atlantic cod (Gadus morhua L.) viscera as components of microbial growth media. Proc. Biochem., 40: 3714-3722.

Beaulieu, L., Desbiens, M., Thibodeau, J. and S. Thibault (2009). Pelagic fish hydrolysates as peptones for bacterial culture media. Can J Microbiol., 55(11): 1240-1249.

Changdao, Mu, Wei, Lin, Mingrang Zhang and Qingshi Zhu (2003). Towards zero discharge of chromium-containing leather waste through improved alkali hydrolysis. Waste Management, 23(9): 835-843.

Einersona, N.J., Stevensa, K.R. and W.J. Kao (2002). Synthesis and physicochemical analysis of gelatin-based hydrogels for drug carrier matrices. Biomaterials, 24: 509-523.

Ghorbel, S., Souissi, N., Triki-Ellouz, Y., Dufossé, L., Guérard, F. and M. Nasri (2005). Preparation and testing of Sardinella protein hydrolysates as nitrogen source for extracellular lipase production by Rhizopus oryzae. World Journal of Microbiology and Biotechnology, 21(1): 33-38.

Green, J.H., Paskell, S.L. and D. Goldmintz (1977). Fish peptones for microbial media developed from red hake and from fishery by-product. J. Food Protec., 40: 181-186.

Horn, S.J., Aspmo, S.I. and V.G.H. Eijsink (2005). Growth of Lactobacillus plantarum in media containing hydrolysates of fish viscera. J. Appl. Microb., 99: 1082-1089.

Langmaier, F., Mladek, M. and K. Kolomaznik (2001). Collagenous hydrolysates sources of proteins. Int. J. Cosmet. Sci., 23: 193-199.

Langmaier, F., Mladek, M. and K. Kolomaznik (2002). Isolation of elastin and collagen polypeptides from long cattle tendons as raw material for the cosmetic industry. Int. J. Cosmet. Sci., 24: 273-279.

Lund, B., Norddahl, B. and B. Ahring (1992). Production of lactic acid from whey using hydrolyzed whey protein as nitrogen source. Biotechnol Lett, 14: 851-856.

Mondal, N.C., Saxena, V.K. and V.S. Singh (2005). Impact of pollution due to tanneries on groundwater regime. Current Science, 88(12): 1988-1994.

Morimura, S., Nagata, H. and Y. Uemura (2002). Development of an effective process for utilization of collagen from livestock and fish waste. Proc. Biochem., 37: 1403-1412.

Moskowitz, Roland W. (2000). Role of Collagen Hydrolysate in Bone and Joint Disease. Seminars in Arthritis and Rheumatism, 30(2): 87-99.

Ohtsuka, K. (1973). Amino acid seasoning produced from scraps obtained from chrome tanned leather. Japan Patent, 7,329,145,1973.

Pachence, J.M. (1996). Collagen-Based Devices for Soft Tissue Repair. J. Biomed. Res. (Applied Biomaterials), 33: 35-40.

Parrado, J., Millan, F., Hernandez-Pinzon, I., Bautista, J. and A. Machado (1993). Sunflower peptones: Use as nitrogen source for the formulation of fermentation media. Process Biochem, 28: 109-113.

Reissbrodt, R., Beer, W., Muller, R. and H. Claus (1995). Characterization of casein peptones by HPLC profiles and microbiological growth parameters. Acta Biotechnol, 15: 223-232.

Stephens, N.L., Bough, W.A., Beuchat, L.R. and E.K. Heaton (1976). Preparation and evaluation of two microbiological media from shrimp heads and hulls. Appl Environ Microbiol., 31(1): 1-6.

Taskin, M. and E.B. Kurbanoglu (2011). Evaluation of waste chicken feathers as peptone source for bacterial growth. J Appl Microbiol., 111(4): 826-834.

Taylor, M.M., Diefendorf, E.J., Brown, E.M. and W.N. Marmer (1993). Enzymatic processing of materials containing chromium and protein. US Patent 5,271,912.

Taylor, M.M., Diefendorf, E.J., Na, G.C. and W.N. Marmer (1992). Enzymatic processing of materials containing chromium and protein. US Patent 5,094,946.

Vázquez, J.A., Ma, P. González and M.A. Murado (2004). A new marine medium. Use of the different fish peptones and comparative study of the growth of selected species of marine bacteria. Enz. Microb. Tech., 35: 385-392.
 
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Asian Journal of Water, Environment and Pollution, Electronic ISSN: 1875-8568 Print ISSN: 0972-9860, Published by AccScience Publishing