AccScience Publishing / ITPS / Volume 2 / Issue 1 / DOI: 10.26689/itps.v2i1.744
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RESEARCH ARTICLE

Antimicrobial Potential of Andrographis paniculata Conjugated Gold Nanoparticle

Nurulshima Razalli1 Subash C. B. Gopinath1,2* Farizul Hafiz Kasim1,3 Ahmad Radi Wan Yaakub1 Periasamy Anbu4
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1 School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis, Malaysia
2 Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia
3 Centre of Excellence for Biomass Utilization, School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis, Malaysia
4 Department of Biological Engineering, College of Engineering, Inha University, Incheon 402-751, Republic of Korea
INNOSC Theranostics and Pharmacological Sciences 2019, 2(1), 744 https://doi.org/10.26689/itps.v2i1.744
Submitted: 23 May 2019 | Accepted: 4 July 2019 | Published: 17 July 2019
© 2019 by the Authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution -Noncommercial 4.0 International License (CC-by the license) ( https://creativecommons.org/licenses/by-nc/4.0/ )
Abstract

Background: Herbal extracts have been traditionally used as antibacterial agents, and different strategies have been 
proposed to enhance their antimicrobial activities. This research aimed to assess the antimicrobial potential of Andrographis paniculata conjugated gold nanoparticle (GNP) compounds against Escherichia coli and Bacillus subtilis.
Methods: Herbal extract was conjugated with GNP through electrostatic interaction, and characterization was carried out by Fourier-transmission infrared spectroscopy, screening electron microscopy, and transmission electron microscopy and supported by energy-dispersive X-ray spectroscopy. Antibacterial activity of the herbal extract and GNP conjugation was evaluated by disc diffusion assay.
Results: Chemical and morphological characterizations of the GNP and herbal extract conjugate revealed intactness of the GNP. In disc diffusion assay, the inhibition zone formed by these compounds was measured for both microorganisms, and they were in the range of 0.6-0.9 cm for the herbal extract. When the herbal extract conjugated with GNP, the zone was between 0.8 and 1.1 cm.
Conclusion: This study demonstrated the potentiality of GNP to carry the antibacterial compounds from the herbal extract.

Keywords
Andrographis paniculata
Gold nanoparticle
Antimicrobial potential
Herbal extract
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Conflict of interest
The authors declare no conflict of interest.
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INNOSC Theranostics and Pharmacological Sciences, Electronic ISSN: 2705-0823 Published by AccScience Publishing